Image 1 and 2 (F8 P 100x). Possible diatoms. No idea about ID
Image 2. (F3 P 20x). Preliminary, order Naviculales Gyrosigma sp
Image 3 (FV 40X). Preliminary ID as Glenodinium sp
Image 4 (F9 100X) and Image 5 (imagen1). Same microalgae. I don´t have a clue about it clasification
Dear Francinie Murillo Vega
You should pure each algal isolates then go for SEM for morphological analysis and then their identity confirm with 18Sr DNA sequencing, the obtained sequence should copy and paste on the NCBI data base and BLAST it. Then you will found the correct identity of your desired interesting algae.
From a rough look, it looks like all images are Peridinium sp or Glenodinium sp (genus Pyrrophyta/Dinoflagellates) except for the second picture, which may be Nitzchia sp or Navicula sp (diatom). Yes, it's quite difficult to differentiate between them if it's merely through a light microscope. You may want to check by starting with identification keys and refer to algal sites such as algaebase.org. You can further confirm it using SEM, TEM following the procedures.
F3 is Pleurosigma sp
F4 is Scripselia sp
The other three are deficult to identify needs clear photo
Best
The first photo is likely a Paulinella, a type of thecate amoeba, not an algal species. The second is likely a Pleurosigma or Gyrosigma species. The third photo is likely a Scrippsiella species. Photos 4 and 5 depict cells of some kind that did not preserve well with your fixative or if live were damaged by the heat from the microscope's light.
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