I want to study the trend of a variable (say firms' investment in R&D) across four waves of a large-scale cross-country survey. Nevertheless, firms surveyed in one wave may not be the same of the following one, altohough their characteristics (size, nationality, etc...) are similar.

Am I allowed to make t-tests on means between independent samples from one wave to another and discuss a trend accordingly? Do you know of similar approaches in the business or innovation literature?

Thank you very much,

Marco

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