I am working on the Alzheimer's disease and I have done ThS staining and I will be proceeding for the silver staining. Can anyone give me the protocol for the silver staining of a brain section?
a good reference for silver techniques with respect to Brain amyloid deposits is:
Braak H and Braak E (1991). Demonstration of Amyloid Deposits and Neurofibrillary Changes in Whole Brain Sections. Brain Pathology 1:213-126.
We utilized a modified Campbell-Switzer (Pyridine) stain (which is outlined in the above paper)- The key is when developing, especially if you have large mount sections, to rotate the slides midway through developing because the silver will precipitate and you will get gradient staining.
Also, a helpful guide with protocols for overall Amyloid detection (including biochemical):
Rostagno A and Ghiso J (2009). Isolation and biochemical characterization of amyloid plaques and paired helical filaments. Curr Protoc Cell Biol.
Bob Switzer gives a protocol for the stain on the homepage of NSA (neuroscience associates). This will work fine and derives from the developer. What I can add is that you should do the staining procedure itself in temperature controlled environment, e.g. cuvettes in a water bath at 20°C, since the staining is not only dependent on fixation but also on temperature (faster if warmer).
The method used in the Laboratory of Neuropathology at the University of Washington, personally borrowed from a commercial laboratory over 30 years ago, and in the hands of two of our technicians was the Bielschowsky silver impregnation. We use it not only in 10% buffered formalin fixed paraffin embedded tissue, but on frozen brain tissue as well. Let the developer Na Thiosulfate age for 24 hrs before use.
Acta Neuropathol (Berl) 1993;86:645-650).
Additional references can be found in, Luna LG (1968) Manual of histologic staining methods of the Armed Forces Institute of Pathology. American Registry of Pathology, 3rd edn. McGraw-Hill, New York. And, Carlton’s Histological Technique. Fourth Edition, 1976. Revised and rewritten by R.A.B. Drury and E.A. Wallington. Oxford University Press, London, New York, Toronto.
If I can find the protocol I will kindly provide it.