i know that if you want to test potassium flux of a potassium channel in a proteoliposome setting,
people usually have Potassium inside the liposome, and either NaCl or nmdg-cl outside the liposome with cccp and acma present outside. This will allow potassium to go outside of the liposome, which will drive H+ inside and lead to quench of acma and hence to decrease in fluorescence.
I was wondering if I can do the reverse to test it - have nacl inside the liposome and kcl outside the liposome? theoretically it should still work but you would see increase in fluorescence instead of decrease?
basically i wanted to see if I can use same batch of liposomes to test two different ion flux without having to switch what's inside the liposome