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Questions related from Yn So.
i know that if you want to test potassium flux of a potassium channel in a proteoliposome setting, people usually have Potassium inside the liposome, and either NaCl or nmdg-cl outside the...
12 June 2025 3,823 0 View
In many papers that uses proteoliposome uptake assay of proton-coupled transporters (proton symporters), people compare the results of "proton-dependent liposome assay using different pH in...
08 December 2023 2,705 0 View
Hi, When making liposomes, the lipids in organic solvent are first put in a round-bottom flask and are 'rotated' while getting dried under nitrogen gas to obtain "a uniform thin film" of lipids...
11 November 2023 9,934 1 View
Hi, Many membrane protein Cryo-EM structures are in detergent micelle. I was wondering how you would prove that the membrane transporters purified in detergent are still active? because the...
25 May 2023 8,110 2 View
Hi, I'm trying to learn how to do liposome uptake assays but i can't get understand why some papers do "counter-flow" liposome uptake assays while most of the papers don't. By liposome uptake...
16 May 2023 3,878 1 View
Hi, This is probably a very naive question, but I see that a lot of Cryo-EM structure uses FAB to stabilize the conformation or to increase the size of the protein to solve its structure. How can...
15 February 2023 8,973 1 View
01 January 1970 658 0 View
