Yes, you can store it in TBST. If it is for many days, go ahead and put it in TBS or diH2O. These solutions are better for maintaining antibody binding because there is no detergent. Given infinite time in TBST (or any solution), antibodies will eventually dissociate from the target protein.

Hi Vittal Kurisetty

* Blots can be stored in TBS for 1-2 weeks at 4 degree, however for long term storage the membranes should be dried and stored in -20 or -80 freezers in a sealed plastic bag.

* Remember to thaw frozen blots to room temperature before removing them from the bag to prevent cracking.

Thanks,

Thank you so much David and Samir for the help in solving the situation. I sincerely appreciate it. I've stored them before in TBS-(T) after probing them but never stored them without probing with Ab's.

Better to store the WB membrane after drying it (make sure no moisture should not be there) and you can store it in freezer for several days but not too longer.

Thank you so much Sachee for the help in solving the situation. I sincerely appreciate it. You've a great day..