Biological production of hydrogen gas has significant advantages over chemical methods. The major biological processes utilized for hydrogen gas production are bio-photolysis of water by algae,dark and photo-fermentation of organic materials, usually carbohydrates by bacteria. Sequential dark and photo-fermentation process is a rather new approach for bio-hydrogen production. One of the major problems in dark and photo-fermentative hydrogen production is the raw material cost.
Carbohydrate rich, nitrogen deficient solid wastes such as cellulose and starch containing agricultural and food industry wastes and some food industry wastewaters such as cheese whey, olive mill and bakers yeast industry wastewaters can be used for hydrogen production by using suitable bio-process technologies. Utilization of a forementioned wastes for hydrogen production provides inexpensive energy generation with simultaneous waste treatment.
The concept of the "hydrogen economy" was quite popular until people started to ask where all that hydrogen would come from. One possible path is using bacteria, another is solar energy: Penn State researchers have announced a proof-of-concept device that can split water and produce recoverable hydrogen.
The main process for biological production are-
1. Direct bio photolysis by green algae.
2. Indirect bio photolysis cyanobacteria.
3. Photo fermentation by anaerobic photosynthetic bacteria.
4. Dark fermentation by anaerobic fermentation bacteria.