Generally, TBARS Parameter Assay is carried out at 532 nm (sample's contributions to the final absorption) can be done with UV-Vis Spec. Unless Your Sample Volume is Sufficient Enough.
In theory yes. Most plate readers have a very limited spectrum, while UV-Vis spectrometers do not and have an adjustible wavelength.
can you pls send me a protocol
The protocol which I got from r and D systems is using a microplate reader pls suggest me how to modify the same
I don't have a protocol, but you can find a TBA perdure in old versions of the AOAC where they are measuring the peroxide value of a fat or oil spectrophotometrically,
Doesn't matter. But to have to validate the method per CLIA guidelines or ICH Q2(r1).
Review this guideline!
http://www.ich.org/products/guidelines/quality/article/quality-guidelines.html
Yes, you can use spectrophotometer in measurement of OD; BUT with further disadvantages such as:
1. Time-consuming as you need to use blank before measurement OD of each sample.
2. Mistakes during procedures.
3. Difficulties in adjustment of preferable wave length.
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