I noticed that most study on sleep (including sleep deprivation and rebound) use a photoperiod of LD 12:12. In this way, for sleep deprivation, they usually choose to deprive 6, 12, 24h. Is that like a convention we should follow?
For me I want to raise them under different photoperiod, like longday and shortday photoperiod (e.g. LD 11:13, 15:9..). I am not sure whether it is OK. Thanks for your kind and useful answer!
If you systematically change the photoperiod from 12:12 to another photoperiod in certain small increments that can be a useful way to ascertain the impacts of gradual shifts in the day length. Just because certain photoperiods (e.g., 6:18, 24:0, etc.) have been used should not limit you from looking at others. Having said that, you may find it beneficial to include at least one photoperiod that has been included in other studies for comparison purposes. But ultimately, it depends upon your specific hypothesis you want to test.
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