What is the open channel conductance of BMIM-Cl?

04 February 2016 7,276 1 View

Which is more accurate approach to calculate ionic current in DNA translocation simulations using GROMACS ?

I am studying translocation of dsDNA poly(AT)45 via graphene nanopore at 0.1080 V/nm electric field in 1 M KCL solution. The production run is performed in NVT ensemble using Nose-Hoover...

31 May 2015 2,610 4 View

How can one assign failure properties of RCC slab in abaqus while analysis?

08 February 2015 1,754 3 View

What is the affect of wiind shear effect on aircraft wings?

Can anyone please help me in this problem of reducing "wind shear effect on an aircraft wing"? My friend just initiated this project entitled "transport modelling to reduce wind shear effect on an...

06 October 2014 4,917 5 View

What is the range of acoustic telemetry. Can this be used in wireless sensor networking instead of very high frequency transmitters?

It seems acoustic telemetry has low frequency than rf or microwave. This may not harm the crops in wsn for agriculture farms.

06 January 2014 5,088 1 View

Spectrum anlyser and FFT analyser

What are the exact differences between these types of equipment and their applications

29 June 2011 6,541 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View