Can we use Neubauer's chamber for counting diatoms? or counting 'per high power field' is a better techique for diatom counting?

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Kevin Kennington

You may want to consider making permanent slides as it is likely that you will not be able to id the diatoms to species level using a Sedgewick-Rafter. I would recommend mounting the diatoms in a suitable mountant such as Naphrax which has a suitable refractive index for studying diatoms.

Geddada Mohan Narasimha Rao

As said by Dr Tan, Sedgewick Rater cell is suitable for counting the diatom s, colonial forms and some filamentous forms such as Lyngbya also.

Mandar Sane

Thanks Dr Tan, Dr Kevin and Dr Rao

Abhijit Mitra

Counting only and only diatom is bit problematic