Can we use HPLC-UV for measuring dopamine and serotonin?

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Manju Tembhre

Hi,

Serotonin, dopamine, and its derivatives can be estimated in brain samples by HPLC-UV. Below is the link for your reference:

Article A validated HPLC-UV method and optimization of sample prepar...

Manuel Gesto

this other thread might be of interest:

https://www.researchgate.net/post/HPLC_plasma_catecholamine_and_serotonin_detection

UV detection is not the most sensitive choice for this kind of compounds. It might be possible to use UV for detecting monoaminergic neurotransmitters, but the sensitivity won't be enough to detect their metabolites, which usually have much lower concentrations in neural tissue.

The paper suggested above does not cover the detection of dopamine.

Best of luck with your search

Jeffrey C. Glennon

In principle yes. I've used HPLC-ECD in the past to measure monoamines but HPLC-UV works as well. You should read the Thomas (2015) paper. The DOI is: Article A validated HPLC-UV method and optimization of sample prepar...