I would like to make an oxidative stress model for studying the antioxidant activity of a drug using C6 cells. Can I use the undifferentiated cells for the same.
You can expose cells to:
1) tert-butyl-hydroperoxide (tBHP), hypdrogen peroxide analog
2) Nitric oxide-donating aspirin (NO-ASA) consists of conventional aspirin to
which the NO-releasing moiety – ONO2 has been attached via a
chemical linker
Glimpse into these papers which will give you further details.
Yes. In addition, you may also use 3-Morpholinosydnonimine (SIN-1), which induces Oxidative as well as nitrative stress and peroxinitrate (ONOO-) generator by praticipating in Fenton Reaction in the presence of iron, OH, and NO free radicals. SIN-1 is a highly potent free radical donor to induce Oxidative stress and is implicated in many Neurodegenerative disorders. Good luck with your research.
Respectfully,
Dean Sharma
Haitham Al-Madhagi and Sushil Sharma Sir, Thank you so much for your response. I'm using hydrogen peroxide analog for inducing oxidative stress. My doubt is that the cancer cell line(c6 glioma cell line) is under stress already. Is it suitable or should I go with a normal cell line? or I should differentiate the cancer cell line and then study the antioxidant oxidant activity after inducing oxidative stress?
Yes, you are right. However, it depends on the frequency and intensity of free radical attack. Usually, primary and secondary free radical attack can be attenuated by endogenous, natural, and synthetic antioxidants, which serve as potent free radical scavengers as a sponge system. To analyze, you may use single tailed t-Test to evaluate the Statistical significance of your data.
Yes, you can also use a normal control of a primary cell culture and analyze your data using two tailed t-Test or F-Test depending on the sample size and distribution. I wish, I could write more on this topic.
For details, you may refer to my book The Charnoly Body, A novel biomarker of mitochondrial bioenergetics, CRC, Press, FL U.S.A. Good luck.
Affectionately,
Dean Sharma
Dear colleague! It depends on the characteristics of the agent for oxidative stress and its concentration.
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