Hello Tanushree Paul,

You can use non polar solvents like n-hexane and ciclohexane, also check this work for more información,

Good luck

https://www.sciencedirect.com/science/article/pii/S1383586618340292?casa_token=MqIc7SPHlJ4AAAAA:HJsWmpYVZt9apvMIu7hIsyZpb7qwvjwY4UH5GdMpyfcmv5rNv32vLgNFdhiIDoqAOfDR1LUQpw

Thank You

Dear Tanushree Paul ,

Your question cannot be answered straightforwardly, it all depends on what you want. It is all a matter of definition. First of all, there is distinction between biosurfactant and bioemulsifier, see for example:

Article Bioemulsifiers are not biosurfactants and require different ...

Furthermore, using for example, the Emulsification index E24 requires comparison between various compounds to get a proper idea about the emulsifying properties of your biosurfactant, see for example:

Article Physicochemical Properties of Biosurfactant Produced by Pseu...

So, I think your point needs to be part of a thorough characterization of your biosurfactant and as indicated in above paper be compared with a commercial surfactant and/or another (closely related) well-characterized biosurfactant.

Best regards.

Hexadecane is a ideal candidate to check the EA. why do u want ot use another reagent? when you have an ideal one.