Q1: The brain samples are one month old and are kept in 30% Sucrose solution. Can we process those samples for IHC
Q2: Can we process these sucrose dipped samples for parafin wax block preparation.
Suggestions will be highly appreciable....
Q1. Not a good idea to dip for one month or longer.
Q2. Never try that.
Q1. Are these samples in 30% Suc PBS1x? If the antibody is very good the IHC may work. Although the staining will never be as nice as using freshly fixed tissue.
Thank you Dr Zhou and Dr Alexandre for your valuable suggestions...
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?
24 February 2021 5,602 2 View
what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....
17 February 2021 8,987 5 View
02 February 2021 9,790 2 View
In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...
13 January 2021 9,309 4 View
In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...
01 January 2021 3,271 4 View
Does anyone know how to perform a sensitivity analysis for crop evapotranspiration ( FAO Penman-Monteith equation) using daily meteorological data?
27 December 2020 9,552 3 View
I have modeled the lumped element circuit for the 3D structure which has been simulated in HFSS. Now I need to derive the equations for the lumped elements keeping the S-parameters of the...
23 December 2020 172 3 View
We are doing the prediction of soil nutrients using spectral information by applying machine learning models. More than 300 soil samples were used from one district for analysis. We got the...
21 December 2020 9,663 3 View
I have to compare yield parameters of a tree crop grown under three irrigation methods. But these systems are already established separately in three adjacent fields. Since I can not follow a RCBD...
21 December 2020 3,065 5 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax
28 February 2021 6,685 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...
22 February 2021 730 2 View
I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...
22 February 2021 4,374 3 View
Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...
18 February 2021 1,518 1 View
My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...
10 February 2021 3,580 1 View