Q1: The brain samples are one month old and are kept in 30% Sucrose solution. Can we process those samples for IHC
Q2: Can we process these sucrose dipped samples for parafin wax block preparation.
Suggestions will be highly appreciable....
Q1. Not a good idea to dip for one month or longer.
Q2. Never try that.
Q1. Are these samples in 30% Suc PBS1x? If the antibody is very good the IHC may work. Although the staining will never be as nice as using freshly fixed tissue.
Thank you Dr Zhou and Dr Alexandre for your valuable suggestions...
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