What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

Did anyone do microglia Oil Red O staining?

I need to stain adiposome in microglia, but I don't know how to confirm that are really success? method : Removed media and washed cells with PBS, then added 4 % paraformaldehyde for 20 minutes at...

24 November 2020 2,359 2 View

How do I solve "unrecognized in LdAtmC" of Gaussian 16 l301.exe type error?

Hello, I have trouble with transitional state calculation in gaussian 16 program. I started with the first frequency calculation with cartesian coordination and it ends up normally. the input...

15 October 2020 1,498 1 View

How to heat a reactor up to 900C?

I'm taking a bachelor design, I'm supposed to maintain an endothermic reactor at 900C and 30 bar. The typical jacketed reactor is not really feasible and I'm thinking of fired heater to maintain...

07 October 2020 8,558 5 View

Help: How to install Skynet?

SkyNet is publicly available as an open-source software at https://bitbucket.org/jlippuner/skynet. However, i can not install it on my computer? Have anyone met the similar problem? First, CC=icc...

20 September 2020 6,912 16 View

What is the best format to export from igraph to gephi?

I have been using R, igraph, and gephi to create semantic networks. I generally export gml file from R through igraph, then import the gml file into gephi for further adjustment. However, I am now...

06 September 2020 3,906 4 View

Is there any difference between male and female mice?

I found that many atopic dermatitis research used female mice rather than male ones. I wonder the reason why they prefer female ones.

05 April 2020 9,088 3 View

How the presence of salt affect the water-in-oil Pickering emulsion stability ?

I have prepared the stable water-in-oil Pickering emulsion by using stearic acid-modified CaCO3 nanoparticles. The oil phase is toluene and the dispersed phase is deionized water. I want to use...

21 September 2019 9,938 3 View

Is ot possible to extract RNA from leaf without grinding?

Generally, in my lab, to extract RNA from plant leaf, leople grind the samples with the DEPC treated mortars and liquid nitrogen. However, it takes a long time so I want to skip this stage. Is it...

08 December 2018 402 2 View

How to get the AlphaImager software?

Hello. In my lab, people use the gel-doc from the Alpha Innotech, and the AlphaImager software is intalled in the public computer. However, the OS of this computer is windows 98 SE so that it is...

01 November 2018 5,782 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to design a boost converter for a mppt controller?

I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...

28 February 2021 3,637 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

PMSM Inter Turn Short Circuit fault, Dataset?

Hi, I'm looking for a dataset of a PMSM with an inter turn short circuit fault. Does anyone know a dataset which is available?

21 February 2021 5,663 2 View