Hi, I'm not a specialist of Varroa mites, but you can find interesting explanations + publication at the following website: http://www.coloss.org/beebook/II/varroa/3/2

I hope it will be of help for you

Dear Pierre Huguir ,

you sent me such a nice website link that i can search out every thing about honey bees. this link is very informative and it explained the procedure in detail for rearing mite in laboroatory. thanks a lot

Thank you Pierre for the link. I would visit it too.

Hello Khalid,

Up to now it is not possible to rear V. destructor in an artificial environment. However, it is possible to maintain adult mites under laboratory conditions. You can find some interesting information in this article:

• Article Artificial feeding of Varroa destructor through a chitosan m...

Cheers,

Richard

Dear @ Odemer, Thank you for sharing a very good article. Scientist are continuously working to develop methods for rearing Varroa mite in laboratory condition.

Hello Khalid,

This is a very interesting article, thank you for this. However, it describes maintenance of Varroa mites, not how to rear or breed them artificially. This is, like I mentioned above, not possible up to now. Varroa reproduction is highly host sensitive as described in our work en detail:

• Article Activation and interruption of the reproduction of Varroa de...

But let's be optimistic and see what the future holds for us!

Hi Khalid,

Yes, it is possible. We are established the in vitro rearing of varroa using gelatin capsule.

See the following articles for references:

Comparing four methods of rearing Varroa destructor in vitro

Piou, V., & Vétillard, A. (2020). Varroa destructor rearing in laboratory conditions: importance of foundress survival in doubly infested cells and reproduction of laboratory-born females. Apidologie, 51(6), 968-983.

Egekwu, N. I., Posada, F., Sonenshine, D. E., & Cook, S. (2018). Using an in vitro system for maintaining Varroa destructor mites on Apis mellifera pupae as hosts: studies of mite longevity and feeding behavior. Experimental and applied acarology, 74(3), 301-315.

Piou, V., Tabart, J., Urrutia, V., Hemptinne, J. L., & Vétillard, A. (2016). Impact of the phoretic phase on reproduction and damage caused by Varroa destructor (Anderson and Trueman) to its host, the European honey bee (Apis mellifera L.). PloS one, 11(4), e0153482.