how we can get pure antibodies against one toxin from any bacteria ?
Dear Hassanain,
You can most certainly raise antibodies against a certain toxin if you have identified the epitope(s). I am not sure if that answers your question entirely. You could get either get polyclonals, which are often conjugated with KLH for purification. Or you could get monoclonals, as they are the preferred choice for analytical testing.
Abhishek
Thank you Abhishek for your contribution , of course monoclonal antibody will be better and more specific than polycolonal. but the challenge will be in isolation of pure toxin. do you know any laboratory which can do this part of work commercially?
Dear Hassanain,
I am not aware of any CROs which would specifically purify your toxin. Depending on the organism under study, my guess is a BSL1 should be rather straightforward to find.
Just a suggestion here: if you are doing an ELISA against the whole protein will the purified antigen be required. Otherwise, if you have characterized the epitope already, you could run ELISA against the individual peptide. Sometimes, the purified toxins are themselves available commercially, so maybe you could search for that as well.
What toxin are you talking about? Many bacterial toxins are available commercially and if protein in nature, their genes can be cloned and expressed in suitable protein expression hosts.
I am asking on this "Panton-Valentine leukocidin" (PVL) is a cytotoxin produced by Staphylococcus aureus that causes leukocyte destruction and tissue necrosis
Please go through following paper; it answers many of your questions.
Application of monoclonal antibodies generated against Panton-Valentine Leukocidin (PVL-S) toxin for specific identification of community acquired methicillin resistance Staphylococcus aureus.
http://www.sciencedirect.com/science/article/pii/S0944501314000603
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