What is the optimal section thickness to look at the dendritic branching/morphology?

Hi everybody, I would like to exploring if manipulation of a specific gene of interest in female mice PFC alters dendritic branching/morphology. I have never done this before and was wondering...

01 April 2019 5,133 1 View

What is role of electron density and bandgap of catalyst in activity/ reaction ?

catalysis, semiconductors

07 December 2017 9,422 2 View

Can anyone help me identify this gastropod from brackish environment?

06 September 2016 2,199 2 View

Does anyone have any suggestions on troubleshooting for transferring with western blot?

The problem that I'm having is not getting a heavy Ponceau stain OR detecting bands after I complete Western blot (addition of primary, secondary and chemi-luminescence) after transferring onto a...

23 February 2016 5,461 9 View

Does anybody know two (closely) related insect species with very different developmental times of their eggs?

Does anybody know two (closely) related insect species with very different developmental times of their eggs?

02 November 2015 2,953 11 View

Considering this paper is from 1987, is it still valid to use as a reference in measuring database operations?

20 January 2015 6,331 3 View

My raw264.7 cells are not differentiating into osteoclasts using abcam's RANKL -ab129136. Has anyone used this or has any recommendations for diff.?

Before purchasing abcam's RANKL (ab129136), I contacted the company as to whether a crosslinking antibody was necessary because I didn't want to use one. They assured me that I did not need one....

23 November 2014 522 9 View

Can anyone tell me how to deal with continuous numbers data on SPSS?

Spss

17 December 2013 2,497 8 View

Has media replaced the role of parents, all learning , and socializing processes dependent on media.

Over dependency on media

10 December 2013 7,330 5 View

Are parents more dependent on media to provide entertainment to their children?

Media dependency theory

10 December 2013 9,680 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

No title

22 February 2021 9,932 11 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View

A film coverage that allows for transmittance of wavelengths on the infrared scale?

I've landed on needing some type of highly optically transparent film or cover material that prevents evaporation of culture media; I am not sure which subfield of materials science would be able...

08 February 2021 5,760 1 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View