Discrepancy protein levels and receptor expression?

I measured the expression of a protein that is a receptor in a panel of cell lines. When I measured the protein levels by Western blot the protein levels were about the same in all cell lines ,...

09 September 2020 6,671 3 View

Can I insert sequence (200bp-800bp) in pUC57 plasmid for PCR and qPCR applications??

I have to PCR assays. I want to prepare positive controls with DNA synthetic. But, I'm not sure use pUC57 plasmid. My PCR product are between 200 -800 bp. What other considerations should I have?

02 February 2020 4,585 2 View

Can focused ion beams be used for cutting 500nm wide trenches in metals, and ceramics?

Does anyone have relevant info on this?

01 May 2019 1,045 3 View

What is the geometry of photometry bench baffle?

In a typical photometry bench (light source at one, photometer under test at the other), is the geometry of the baffle critical? If so, how does one design such baffles? EDIT. Thank you all for...

27 April 2018 4,774 3 View

What is the origin of the dots that appear in the SEM pictures of InP or InGaAsP layers after an FIB cut?

I am doing some FIB cuts and analyzing my layer stack. I always see dots in the InP and InGaAsP layers, but I don't know their origin. These dots do not appear when I do a SEM of a cleaved sample,...

27 February 2018 1,151 2 View

How to calculate the surface coverage of two adjacent but no overlapping cellular structures?

Dear all, I am staining two adjacent cellular structures solids with fluorescent antibodies (Alexa-488 and 555). I want to calculate the coverage of the small solid respect to the big one. In...

12 February 2018 2,606 3 View

What is the origin of the dots that appear in the SEM pictures of InP or InGaAsP layers after an FIB cut?

I am doing some FIB cuts and analyzing my layer stack. I always see dots in the InP and InGaAsP layers, but I don't know their origin. These dots do not appear when I do a SEM of a cleaved sample,...

01 February 2018 4,079 2 View

Why do we add sucrose in the buffer used for aldehyde reductase extraction?

During aldehyde reductase extraction, the buffer we use contains sucrose, betamercaptoethanol and EDTA. why sucrose?

22 August 2017 4,567 1 View

What is the best effect size estimator for ecological data with low sample size?

13 May 2017 8,162 4 View

How to quantify DNA before and after amplification?

I run a PCR with the sputum product which was microscopy positive, but after PCR the result was negative.

03 March 2017 9,847 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View