Can we go back from Xylene to DAB?

09 September 2017 1 7K Report

Can we go back from Xylene to DAB? I mean to say I used my slides for staining with a particular Ab and visualizing it with DAB, then counter stained with Cresyl violet. Now they are in Xylene. I can see my specific anbtibody staining but its way too light. So can I restain it with DAB ?

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Science method

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