Can we fix cells with 4% paraformaldehyde after staining the cells with TMRE to study mitochondrial potential?

More Deepanshu Maheshwari's questions See All

Estimation of protein in sample?

I began by processing a 500 mg plant sample, grinding it in 5 ml of phosphate buffer. Following this, I centrifuged the mixture for 15 minutes and collected 1 ml of the resulting supernatant. To...

04 April 2024 4,030 1 View

Has anybody used Matlab for size distribution analysis of aggregated particle for SEM ( Scanning electron Microscopic ) image?

I have a sem image and I want to know the particle size distribution in the image field.

24 October 2023 2,304 5 View

Can I start a research on behavioral finance on non investors ?

i want to start a research on behavioral finance for non investors. so were i can collect the data why they are not investing or do they want to start the investments and why it is? and I didn't...

20 July 2023 6,814 6 View

What can be the magnitude of GWs produced by cosmic rays?

Gravitational waves that are detected by the current detectors are of long wavelengths and are hence low on energy, but during the initial phase of the universe high energetic phenomena like...

03 July 2023 3,132 3 View

What were the modes of creation of GWs in the early universe?

As we know that high energy phenomena like black hole mergers, neutron star mergers can create ripples in the spacetime continuum but what were the modes of GW creation during the initial phase of...

30 June 2023 8,945 1 View

How to start on Research Design and Sampling Techniques in Cyber Security & Cryptography ?

I need to understand what & how to choose sampling techniques I should pick to explore cryptography issues in cyber security domain.

16 May 2023 8,963 5 View

Should I test a moderation effect if my initial hypothesis is not supported?

In my study , I hypothesized X to have a negative effect on Y and that the moderator W would moderate the negative relationship between X and Y. However, my initial hypothesis of X having a...

15 December 2022 5,392 2 View

Suggestions for open-source or professional software/code for velocity and attenuation based ultrasonic tomography of solid materials ?

I am looking for a software/code (either open source or licensed) that can facilitate attenuation or velocity algorithm based full-field ultrasonic tomography, based on multi ray path...

03 January 2022 9,353 3 View

I am trying to simulate a planar connector in Abaqus. I made 2 parts, a thin 3D sheet, and a cylinder. How can I use a planar connector between them?

My attempt was that I created 2 Reference Points at the centers of the two base of the cylinder and made a wire between them. Using that wire, I made a planar connector. But when I Provide...

22 July 2021 3,977 1 View

How will an optimization algorithm search inside an already trained ANN in Python??

Can someone help me on how to link an already trained ANN with an optimization algorithm in Python. I am finding a lot of stuff online on training an ANN using optimization algorithm. But I don't...

13 July 2021 7,633 1 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View