Can we extract material and dilute Trolox in water in TEAC III assay?

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Why does absorbance drop down over time when using PBS to dilute ABTS?

I use Photphate buffer saline (PBS) instead of ethanol to avoid the precipitation of pectine but when I diluted ABTS+ in PBS to reach 0.7A (+/-0.2A), the absorbance drop down over time. I don't...

08 September 2015 7,829 1 View

How can we store ABTS+ solution longer?

ABTS+ solution can store 2-3 days at room temperature. How can we extend the shelf life of ABTS+ solution? Thanks for any help.

07 August 2015 6,402 1 View

Could anyone help me answer some questions about TEAC III?

ABTS working solution was prepared by diluting stock solution in ethanol or PBS until its absorbance at 734 nm was 0.70 ± 0.02. When do we use pbs and when ethanol? Does material affect to the...

07 August 2015 7,257 0 View

How can we reach absorbance of 0.70 at 734 nm when we prepare ABTS+ solution in TEAC III assay?

The working solution was prepared by taking a volume of the previous solution and diluting it in ethanol until its absorbance at 734 nm was 0.70 ± 0.02. The solution was also pre-equilibrated at...

07 August 2015 7,542 9 View

Why do we need to adjust pH level of canned coffee?

Hi everybody, I found in a canned coffee that they using sodium hydrocarbonate to adjust the pH level. So what is their purpose? I would like to thank you for your attention, if you have any idea...

01 January 1970 8,961 3 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View