Huy Nguyen

6 Questions 8 Answers 0 Followers

Questions related from Huy Nguyen

Why does absorbance drop down over time when using PBS to dilute ABTS?

I use Photphate buffer saline (PBS) instead of ethanol to avoid the precipitation of pectine but when I diluted ABTS+ in PBS to reach 0.7A (+/-0.2A), the absorbance drop down over time. I don't...

09 September 2015 7,807 1 View

Can we extract material and dilute Trolox in water in TEAC III assay?

According to some articles, they extract material and dilute Trolox in acetone. Is it required to use the same solvent and can we use water instead? Thanks for your help.

08 August 2015 3,642 2 View

Could anyone help me answer some questions about TEAC III?

ABTS working solution was prepared by diluting stock solution in ethanol or PBS until its absorbance at 734 nm was 0.70 ± 0.02. When do we use pbs and when ethanol? Does material affect to the...

08 August 2015 7,237 0 View

How can we reach absorbance of 0.70 at 734 nm when we prepare ABTS+ solution in TEAC III assay?

The working solution was prepared by taking a volume of the previous solution and diluting it in ethanol until its absorbance at 734 nm was 0.70 ± 0.02. The solution was also pre-equilibrated at...

08 August 2015 7,521 9 View

How can we store ABTS+ solution longer?

ABTS+ solution can store 2-3 days at room temperature. How can we extend the shelf life of ABTS+ solution? Thanks for any help.

08 August 2015 6,380 1 View

Why do we need to adjust pH level of canned coffee?

Hi everybody, I found in a canned coffee that they using sodium hydrocarbonate to adjust the pH level. So what is their purpose? I would like to thank you for your attention, if you have any idea...

01 January 1970 8,940 3 View