I want to extract a signal of given protein from FTIR spectral data of mixture of proteins. As the IR signature of different chemical molecules are different, so will the signature of a protein be also be different.
We have done such an experiment for the very ideal case of a mixture of lysozyme (LYZ, a-helix is dominant secondary structure) and concanavalin A (CONA, b-sheet is dominant structure). We adsorbed both proteins from their binary mixture on positively or negatively charged surfaces. Even qualitatively one could see, which of the proteins was dominantly adsorbed (CONA: 1630 maximum, LYZ: 1650 maximum).
The quantitative protein compositions were determined by factor analysis on the spectra of the binary protein layers and we used the spectra of the pure proteins as factor spectra. You can find this study under this reference:
M. Müller, B. Keßler, N. Houbenov, K. Bohata, Z. Pientka, E. Brynda,
pH dependence and protein selectivity of poly(ethyleneimine)/poly(acrylic acid) multilayers studied by in-situ ATR-FTIR spectroscopy,
Biomacromolecules, 7(4), 1285-1294 (2006)
Best regards,
Martin
We have done such an experiment for the very ideal case of a mixture of lysozyme (LYZ, a-helix is dominant secondary structure) and concanavalin A (CONA, b-sheet is dominant structure). We adsorbed both proteins from their binary mixture on positively or negatively charged surfaces. Even qualitatively one could see, which of the proteins was dominantly adsorbed (CONA: 1630 maximum, LYZ: 1650 maximum).
The quantitative protein compositions were determined by factor analysis on the spectra of the binary protein layers and we used the spectra of the pure proteins as factor spectra. You can find this study under this reference:
M. Müller, B. Keßler, N. Houbenov, K. Bohata, Z. Pientka, E. Brynda,
pH dependence and protein selectivity of poly(ethyleneimine)/poly(acrylic acid) multilayers studied by in-situ ATR-FTIR spectroscopy,
Biomacromolecules, 7(4), 1285-1294 (2006)
Best regards,
Martin
Dear Martin,
Thanx a lot for such insight full knowledge....I am currently referring to the paper you have quoted....
Best regards,
Prasoon
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