Dear researchers,
What are the detection methods for Estrogen hormone in the aqueous solution?
Can we use the UV-visible spectroscopy for Estrogen hormone (E1, E2 or EE2) detection? What is the suitable wavelength?
Best regards
Hi,
Are the concentrations of estrogen you'd like to determine that in (rather) pure solutions?
If so, you can use UV spectroscopy. To determine a suitable wavelength, you can acquire an absorbance scan over the wavelength range that the spectrophotometer covers; look at the wavelength(s) at which estrogen absorbs the most.
Then you can acquire a calibration curve by using solutions with an increasing concentration of estrogen and determining the absorbance at the selected wavelength.
If you now acquire the absorbance at that specific wavelength for an estrogen-containing solution of unknown concentration, you can use your calibration curve to calculate back the estrogen concentration. Make sure that the matrix of solutions used for your calibration curve matches that of the unknown sample, in terms of buffer strength, pH, etc.
Dear Eef,
Thank you so much for your reply. I greatly appreciate your help. The concentrations are from 0 to 10 ng/L in pure water at a pH of 6.8-7.2. I use phosphate salts to prepare the buffer solutions. What is the range of wavelengths you propose to me?
Kind regards
Foroogh
Please look at the following below attached files which may help you in your analysis.
Thanks
Dear Islam,
Thank you for answering the question. I already studied them. However, I do not have the necessary devices such as chromatography columns to use those methods of detection. That's the reason I want to find another detection method.
Best regards
Foroogh
- Badges
- Science topic