No

yes, you can but would need some fluorescent probes & equipment (e.g. plate reader or microscope), depending on your model: Article Imaging of oxygen and hypoxia in cell and tissue samples

Many thanks for rapid response

Yes, but first you need accurate, real-time measurement of O2 in your culture, and not just the 3% or 5% reading on the incubator. That tells you what the gas-controlled incubator is aiming for in the atmosphere, but it does not tell you what the pO2 is on the cell culture surface, which even at the same O2 setting can vary widely from flask to flask. Get the Petaka G3 cell culture cassette. It not only autoregulates the amount of oxygen dissolved in your culture media, without the need for tri-gas incubators or external CO2 supplementation, it can be used to precisely measure and record pO2 in your media in real time. Then you can correlate the pO2 and oxygen culture history of those cells with another experimental measure. All without introducing any foreign probes or harming your cells in culture.

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