Can we club together immunofluorescence(Alexa fluors) with Eosin staining ?

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Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

What is meant by baseline of FTIR data?

I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...

03 August 2024 6,070 3 View

What is Random Audit?

HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...

31 July 2024 5,309 7 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Does anybody have Anti-Claudin8 and Anti-Claudin2 Primary conjugated Ab (Target Species: Human)?

I need Anti-Claudin8 Primary and Anti-Claudin2 conjugated Ab for my immunofluorescence experiment. If anybody has worked on it. Please share the accession number/details.

14 July 2024 485 0 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View

Eliminate the autofluorescence of red blood cells in mouse spleen paraffin sections?

Immunofluorescence staining of mouse spleen paraffin sections showed strong fluorescence signals in red blood cells in the control section without antibodies. Is it autofluorescence of red blood...

02 July 2024 827 3 View

immunofluorescence, False Positive Nuclear Staining in Hydrogel-Embedded Breast Cells?

I have primary breast cells embedded in the hydrogel. When stained with EpCAM without the hydrogel, the staining was correctly localized to the membrane as expected! However, after embedding in...

28 June 2024 353 1 View

Immunofluorescence of brain tissue?

I did a frozen section of mouse brain tissue with immunofluorescence staining. My partner and I did c-fos staining together. We used different primary and secondary antibodies for different...

20 June 2024 8,989 1 View

How to fix cells with glutaraldehyde for immunofluorescent staining?

Hello, I am trying to fix primary cells with glutaraldehyde. I am trying to stain cell membrane proteins and I have not been successful with PFA and methanol fixation. Does anyone have a protocol...

17 June 2024 3,434 5 View

Hi All, about multiplex immunofluorescence,what could be the reasons for unwated flurophore background ? other than autofluorescence and 1ry Ab conc?

Could the tissue quality/age be an issue ??

15 June 2024 7,763 0 View

Hello, could you please give me details of the antibodies that can be used to perform quantitative immunofluorescence of AT1Rs in the mouse brain?

Hello, could you please give me precise details of the antibodies that can be used to perform quantitative immunofluorescence of AT1Rs in the mouse brain?

13 June 2024 3,591 1 View