Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

What is the purpose of internal control and extraction control in q PCR procedure?

Is extraction control also add to the qPCR mixture with the sample or does it run separately? Can anyone please explain their importance and how to use them in q PCR protocol? Thank you.!

23 November 2020 314 3 View

Can anyone explain the difference between recombinant proteins vs recombinant chimeric proteins?

Difference and advantages and disadvantages of using recombinant proteins versus chimeric proteins as antigens in serological tests

28 September 2020 5,307 3 View

Is there any possibility to detect both IgG and IgM antibodies in the same ELISA plate?

I am looking forward to developing an ELISA which can catch both anti-Toxoplasma IgG and IgM antibodies in Marsupials. Has anyone have any thoughts about detecting two different antibodies ( IgG...

24 July 2020 4,193 3 View

What are the data that can be extracted using contour maps?

To identify factors affecting landslides, I want to know what data can be extracted using contour map of a certain area. For an example slope of a land can be found. Can factors like geology,...

19 March 2019 6,187 15 View

Can you give suggestions for a research based on GIS and predictions?

I'm in search of research topics for my 4th year final project. I'm interested in doing my research related to GIS and predictions. I would be glad if you can help me by giving some ideas.

10 February 2019 5,839 9 View

How to convert SNP data in microsoft excel into hapmap?

I have a big data set of SNP in excel format, how can i convert them into hapmap

01 March 2018 2,605 1 View

Can someone suggests good molecular markers for population genetics of leafy liverworts?

i'm looking for good molecular markers on populaion genetic studies of leafy liverworts

27 February 2017 6,106 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View