Can we add more than 1 gene into plasmid vector?

More Aysegul Yalcın's questions See All

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

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VIF problem in PLS-SEM?

I have VIF values that greater than 5 (exactly 5.6) in my PLS-SEM results. Would it be a problem to be accepted into the SCI/SSCI journal?

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How Can I find a reverse primer with good rating, if I need to amplify entire gene?

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How to measure purified protein concentration for SDS-page?

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How can cyclic symmetry be defined in dynamic, implicit analyse in Abaqus?

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I need to distillate my plasmids concentration but I dont know how can i calculate it ?

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How can I prepare AESO as a resin for composite production ?

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Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

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PUC19 as a transfer plasmid for lentiviral delivery?

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Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

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How to use protein A G beads to purify antibody?

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Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

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I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

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Does the property of GFP affect a fusion protein's localization?

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How much amount of DNA (ligation mix) should I use for transformation?

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15 February 2021 9,563 6 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

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Sathish Dorairaj

Hi Aysegul Yalcın ,

Yes. If the vector of interest has enough capacity, two or more genes can be inserted in to a vector. The efficiency of gene expression will be better if each gene has separate promoter and terminator.

Best wishes.

Md. Firoz Alam

You can follow Gibson assembly. For details,

https://international.neb.com/products/e5510-gibson-assembly-cloning-kit#Product%20Information