We have resolved a structure of epimerase that catalyzes the epimerization of β-methyl-indolepyruvate. A Histidine has been identified as a proton acceptor and the indole ring of substrate makes it non-rotatable in the pocket. However, there is not any other residue could be enlisted as proton donor in the pocket. Is it possible that water molecule could be employed as proton donor for substrate reprotonation?
Here is a paper on an epimerase that mentions the likely role of water in the catalytic mechanism.
Article Catalytic Mechanism and Mode of Action of the Periplasmic Al...
Here is a review article on epimerases that also mentions the role of water:
Article Epimerases: Structure, function and mechanism
Water has wonderful properties it can be proton donor and proton acceptor, oxidizing agent and reducing agent, acid and also base....... So the chemistry of water is very intresting
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