Bin Liu

4 Questions 10 Answers 0 Followers

Questions related from Bin Liu

Can water act as proton donor during the catalytic process of epimerization?

We have resolved a structure of epimerase that catalyzes the epimerization of β-methyl-indolepyruvate. A Histidine has been identified as a proton acceptor and the indole ring of substrate makes...

03 March 2019 6,003 3 View

How to calculate Km and Kcat in multi-enzyme reaction?

I want to get the Km and Kcat of enzyme E3. However its substrate C is very unstable for its R-S conformation exchange.

02 February 2018 3,976 4 View

Can these prove my protein aggregation was induced by disulfide bond?

Though the samples were treated with TCEP and 1M Arg, protein aggregation still can be observed by NMR after 2-3 days.To figure out why, I ran SDS-PAGE with different sample...

04 April 2016 5,885 14 View

It make me crazy! Who can tell me what happend to my E.coli?

strain: Rosetta(DE3,plysS) plasmid: pet28a medium: M9 medium with 50ug/ml kanamycin and 34ug/ml chloramphenicol After incubation in LB(kanamycin, chloramphenicol) at 37℃ over night，transfer 10ml...

02 February 2016 2,877 10 View