One-Way or Two-Way ANOVA?

Dear, In the preparations for publishing a manuscript, we have a big argument about the proper analysis of variance to compare multiple groups for a certain staining quantification. We have two...

27 December 2020 7,916 3 View

What is the full script that I can use to extract sequences from fasa file by using sequenc IDs in text file using python 3 in pycharm environment ?

I want to extract sequences from fasta file using a text file witj ids in python

29 May 2020 1,674 3 View

Measure the Real area when tilt SEM stage?

03 April 2020 7,068 3 View

How to praper graphitic carbon nitride from urea ?

I want to know How to praper graphitic carbon nitride from urea ? I need a proven method because I tried several methods that didn't work

27 October 2019 7,236 3 View

Why cell proliferation increased after change amino acid by SDM?

Dear Scientists, I am working with a gene of interest in colorectal cancer, which has no role in cell proliferation. In addition, by knockdown this gene, the FAK and SRC protein expression are...

09 October 2019 6,257 3 View

Can Single Cell Sequencing be performed on any species? or Human and mouse genomes are the only ones available?

Can single cell sequencing be performed on cells from species other than human or mouse?

15 July 2019 3,840 1 View

How to submit your research manuscript to more than one publisher at the same time?

I found an interesting web site that allows the researchers to legally submit their manuscript to more than one publisher at the same time. The publishers will have initial screening and come...

19 December 2018 3,906 13 View

How to prepare a conditioned media post transfection with specific plasmid ?

Hi Researcher, I am new PhD student and I have plan to start an experiment which is requires to do different techniques. Therefore, I would like any researcher find my post to answer my question...

20 May 2018 9,526 2 View

Can I detect amino acids by biuret reagent during separation on agarose gel electrophoresis?

During separation of amino acids using gel electrophoresis, can I use Biuret reagent or Ninhydrin.

14 November 2017 6,552 4 View

Health financing strategies to universal health ?

Does any one have studies or articles about developing health financing strategies to assist the implementation of universal health coverage Thanks

03 April 2016 7,515 9 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Can we use a compensation matrix with single staining that the negative population for each single staining is below zero?

Recently we used single staining to make a compensation matrix. But, for each single staining we saw that 13 to 25 percent of our negative population had been shown below Zero. In this situation...

21 February 2021 9,982 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View