I'm planning a project to study the peri-infarct region of a stroke using brain slices made post-MCAO. Is there any reliable way of assessing the peri-infarct in live brain slices - i.e., how far from the edge of the core can be called the peri-infarct and when would the 'unaffected' tissue begin? Are there any tell-tale signs in the tissue that would help identity it? Could this region be live-stained in any way? Of course TTC staining is the gold standard of defining the stroke infarct, but the stained tissue then cannot be used in live physiology experiments.. So some other way of knowing where the core, peri-infarct and normal tissue are in slices made from brains exposed to transient stroke in vivo would be very useful.
Any suggestions would be helpful - thanks in advance!
Hy,
perhaps you could try Propidium-iodide (PI) stainings.
I organotypic slice cultures is is very commonly used to stain dying/dead cells.
Kind regrads
Stefan
Dear Anusha Mishra
I am also working on MCAo and BCCAo models of stroke in rats and mice respectively. The possible way to sort out your problem is after sacrificing and removing of brain make two slices of the brain one anterior to brigma and other posterior to brigma. Then use one of your brain slice for TTC staining and you can use other for your physiological experiments. After TTC stain you can easily observe the peri-infarct area based on intensity of brick red color. The infarct tissue you can see whitish color, the live tissue will appears brickesh red color and the Per-infarct you can see mild white as well as brick red transformations. Then you can consider the same region as peri-infarct region in unstained brain slice also.
Best of Luck,
Regards
Thank you Stefan and Dibbanti. These are both great suggestions and I will be trying them.
Best,
Anusha
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