Please I need an answer:
I have a treatment contain enriched compost with microbes. I need to evaluate the microbes but don't want to proceed a CFU. Can I use the t60 uv vis spectrophotometer ?. And if yes what procedure to follow?
Need to clarify whether you want to quantify microbial population or microbial activity?
@ Hanan, the answer is Yes you can measure. For example, a wavelength of 600nm (A600) is used for measuring bacterial concentration.
@ Hanan, if you want to measure microbial biomass that also you can do. A rapid and inexpensive method to estimate SMB based on the near UV light absorption of certain molecules (nucleic acids/nucleotides) of microbial cells extracted from CHCl3 fumigated soils . The UV absorbance at 280 nm is significantly correlated to SMB measured by CHCl3 method as follows. SMB = 21747 x E280 nm. This is a fast and simple method to determine SMB but uses CHCl3.
Thank you Dr. Tarafdar. yes I want to measure the SMB in the samples. Do I need to fumigate the soil. Seriously, I need the procedure form A to Z since its my first time using t60 uv vis spectrophotometer. If you could support me with a good reference.
Dr. Debnath I need to quantify the microbial activity since it is a fungal and bacterial samples
Yes, you have to do chloroform fumigation. Attached file may provide you details procedure. For microbial activity measurement you may estimate esterase or dehydrogenase activity.
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