Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

Why are we measuring gel content of NBR latex? Does gel content related to the amount of cross-linked in a polymer?

Hi good day to everyone! I am actually studying the gel content of synthetic latex. Refer to ASTM 3616, gel content is measure of insoluble rubbers in the chosen solvent. So, I just would like to...

07 February 2021 605 4 View

How to delete a copy of a paper, and how to delete a co-author's name?

I mistakenly uploaded a copy of the same paper and entered my alternative name. How to delete them out? (I couldn't navigate to the right buttons to do it. So from where to where is what I don't...

07 January 2021 7,694 6 View

What effect will different solvents have on the ATRP reaction?

I want to do an ATRP reaction, synthesize an ATRP initiator, use DMF as a solvent, and mix DMF, BIBB and TEA to turn red. Why does it turn red? Xylene has been used as a solvent before, and there...

04 January 2021 8,034 3 View

Species identification.Who can tell me the scientific names of these Gastropods?

Species identification. I've been doing research on communities of marine macrobenthos recently, but some of them can't be identified, which hinders my research. Who can help me identify these...

14 December 2020 8,357 3 View

Cell shrinkage during ROS assay(H2DCFDA)?

Hi! I'm Yu, studying about health of MSC. These days, I'm doing ROS assay with H2DCFDA which requires 5~30 minutes of staining with PBS or HEPES or HBSS. In our lab, We use PBS w/o Mg/Ca for...

13 December 2020 8,813 9 View

Can I fix the cells after H2DCFDA assay for FACS analysis?

Hi ! I'm Yu, and planning to analyze ROS assay with H2DCFDA(Thermo) on MSCs. A lot of protocols instruct that treat H2DCFDA at specific concentration for 5~30 min, 20~30 min of recovery time...

10 December 2020 973 1 View

How to reconstruct a Lamb wave by a series of pulses superposed?

Lamb wave is useful for solid structure health monitoring, and it consists many modes. Unlike ultrasonic bulk wave, when given a pulse excitation (for example, 5-cycle sinusoidal pulse), only...

08 December 2020 5,440 8 View

How to interpret the significant effect of covariates when conducting a Two-way ANCOVA?

06 December 2020 8,560 4 View

Did anyone do microglia Oil Red O staining?

I need to stain adiposome in microglia, but I don't know how to confirm that are really success? method : Removed media and washed cells with PBS, then added 4 % paraformaldehyde for 20 minutes at...

24 November 2020 2,359 2 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

How to lessen cell growth?

I am working on the 4T1 murine breast cancer cell line. from the first day I bought this cell line, the cell's growth rate is extremely high. I intend to make these cells resistant to a single...

24 January 2021 2,884 3 View

How long I can store plasma in -80 oC before doing Growth differentiation factor-15 assay ? , Is it different when I stored plasma at -20 or -70 ?

I want to do GDF-15 assay on multidrug resistant tuberculosis patient

29 December 2020 6,764 1 View

Biotech final year project for a bioinformatics beginner?

So my last year project is Drug Efflux Pumps and Persistence in Methicillin Resistant Staphylococcus aureus and we gonna focus on persister cells to study the path way of antimicrobial...

09 December 2020 2,574 2 View

Who can i take a smear from inhibition zone?

I used alcoholic extract of peganum harmala seeds as inhibitor to staphylococcus enterotoxin type A gene (SEA). By using diffusion method I need to know how can i take a smear from zone of...

29 November 2020 3,165 3 View

Which of the ff. solvent is more effective to use as a bactericidal agent in antimicrobial testing of staphylococcus species, ethanol or methanol?

The antimicrobial activity testing of staphylococcus species.

02 October 2020 6,996 7 View

How to make 0.4M acetate buffer of pH 4.75 (250ml)?

I am currently working on a project to determine resistant starch content in food samples. For that the procedure mentions to add 0.4M acetate buffer of pH 5.75 to complete the sample digestion....

09 March 2020 2,368 3 View

Why daptomycin fail to kill my S. aureus in liquid culture?

I tried to do MIC tests on my two S. aureus strains, Methicillin-Susceptible S. aureus (MSSA) ATCC 25923 and Methicillin-Resistant S. aureus (MRSA) ATCC 43300. For the MIC test by broth...

02 March 2020 2,043 3 View

Can someone translate this paragraph into plain English?

Hi all, I'm looking into observational studies to try and better characterize treatment-resistant depression. I'm having trouble deciphering the paragraph in bold below, having read it multiple...

19 February 2020 9,823 2 View

How to extract the methicillin resistant staphylococcus aureus from raw milk?

kindly explain the protocol for the initial extraction and identification of S.aureus.

01 February 2020 9,492 3 View