We want to harvest some mouse intestine tissue without any brown stuff in it. The challenge is that we would also like to perfusion fix the animal first, which makes the tissue stiff and contracting. I could not find any protocol around to deal with this issue. Any suggestions?
Hi, a 24 hour fast prior your endpoint should solve your problem to empty the GI tract
Hi Marie Mortreux , thank you for your answer. I actually just tried that, but unfortunately it only partially worked. A 36 hour fast did clear the stomach but the intestine is still partially filled.
Regarding my setup, I basically left the mouse in a cage with only water in it. Do you think I missed anything?
36 hours is a pretty long fast, but mice eat their feces, so if you did not clean the cage when starting the fasting period that might have an impact. When I have to clean the intestines (it was not after PFA perfusion though), I just turn them upside down and use tweezers to empty them and rinse them. Did you try that?
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