I've been trying to expand the CMV strain AD169 in fibroblasts without success. I'm following the protocol provided by the NIH AIDS reagent program: I'm infecting the fibroblasts monolayer using a MOI of 0.01 and 0.1 for one hour and after 10-14 days I don't really see any CPE, the cells usually begin to detach from the plate, but I don't see any clear CPE.

I have tried HFF and an in house fibroblast cell line available in my lab, but none of those have work.

Thanks for any tips you can provide me with!

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