Can someone share a protocol for total RNA extraction from apple (Malus domestica) leaves?

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Simran Bhullar

Hello,

You must have tried some Trizol based methods, it might be better to try doing it the old CTAB way which works for recalcitrant varieties, here is one protocol which might help.

http://www.researchgate.net/publication/226807792

Article Isolation of High Quality DNA and RNA from Leaves of the Car...

Iftekhar Baloch

Thanks Simran Bhullar.

Finally I saw the bands after modifying the CTAB protocol a little bit. Trizol method wasn't working.