Can someone recommend optimal primers for sequencing rat hybridomas?

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Why is cronbach's alpha sometimes calculated for non-unidimensional questionaries?

I've recently come across with several published articles where questionaries composed of more than one section (where every section is meant to gather data for separate variables/dimensions of...

11 November 2020 1,805 7 View

Could you recommend a Journal to publish a literature review about the relationship between globalization and ultranationalism in Europe?

Currently I am finishing the literature review about the relationship between globalization and the resurgence of ultranationalism parties in Western Europe. This is the first part of my Doctoral...

02 October 2020 9,773 5 View

Where to find data for Frequency Regulation and Peak Shaving control signals for intregration of BESS to the utility grid?

I'm looking to study Battery Energy Storage System (BESS) integration to the utility grid for different applications such as Frequency Regulation and Peak Shaving. I would like to learn about...

05 August 2020 2,075 1 View

Glass luminescence after high electromagnetic pulse?

In a Z-pinch test using Pulsotron-3 fusion reactor, I have seen at high-speed camera that a pyrex glass generated a beautiful green light during some milliseconds after the electromagnetic pulse...

18 July 2020 2,488 2 View

Collaboration against COVID-19 based on 3D printing, any JCR journal suggestion?

Good morning, Our university has collaborated during the last three months of the pandemic with the printing of PPEs by means of 3D printing, contributing with more than 26,000 parts in a...

09 June 2020 7,107 4 View

What is the best intelligent algorithm to control engines?

There are PIDs but usually only the Proportional part of the PID algorithm is usually used Mapping systems, as used in diesel engines But make a several layer PIDs is difficult. Map based systems...

06 May 2020 1,065 0 View

How to calculate total electronic cross sections?

Hello, I'm trying to understand photon shielding parameters such as mass attenuation coefficients (MAC's) and effective atomic numbers (Zeff's). I would like to ask how to calculate for total...

08 April 2020 7,893 2 View

How do you deal with lost data?

Given your specific discipline. Have you ever irretrievably lost data of an ongoing research project? How did you handle it? Thanks in advance. (Also, this is my story. A couple of years ago, in...

29 March 2020 297 21 View

Which environmental problems arise from landfill mining ?

Landfill mining techniques arise as one of the available alternatives to mitigate negative environmental impacts from municipal solid waste landfills. High recycling potential generation is one of...

28 March 2020 1,782 10 View

How should a shapefile (.shp) be referenced?

A local fault shapefile prepared by a public academic institution was required to complement a qualitative analysis. It was not possible to access a study or technical report documenting the data...

11 March 2020 4,329 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Abhijeet Singh

I don't understand exactly what you want to do? Either you must know the genomic change which you have introduced or target, and design primers based on that. If not, primer based method would not work I guess.

Javier Gordon Ogembo

we are looking for optimized degenerative primers to sequence rat monoclonal antibodies.