Can someone please suggest me a protocol to check the presence of secondary metabolites in Cyanobacteria using GC-MS?

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No title

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Lin Chau Ming

you need to check if it has some volatile compound. if no, use hplc. If yes, use the same protocol to plant material

Prabha Kumari Tiwari

Lin Chau Ming,thank you for your kind reply. Kindly suggest some research articles for the same.

Antonio Trani

As reported by Audrain et al. 2015 ( Article Role of bacterial volatile compounds in bacterial biology

) tha main volatile metabolites of cyanobacteria are hydrocarbons. Milovanovic et al. 2015 (

Article Determination of Volatile Organic Compounds in Selected Stra...

) studied the VOC released by cyanobacteria. I think that the most sensitive and easy way to investigate and compare volatiles among samples, in every matrix of study, is solid phase micro extraction coupled to gas chromatogray /mass spectrometry. Considering the target compounds I suggest to use the metodoly we described in

Article Lactic acid fermentation enriches the profile of biogenic co...

It is very easy to perform. Good luk.

Antonio

Thank you Antonio Trani for your kind reply.

Kulvir Singh

dear Prabha Kumari Tiwari

this article shall be useful for u.good luck

Thank you Mr. Kulvir Singh for sending me this article.