Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3? or the use of plasmid pET 23B?

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Manuele Martinelli

Dear Sebastian

on my blog: PRoteoCool (https://proteocool.blogspot.com/) at PAGE2, expression you can find some protocols to express protein with BL21(DE3) using high cell density media.

I hope that it can be usefull to you!

good luck

Manuele

Anju Kaushal

Please check the link below:

Article Increased levels of recombinant human proteins with the Esch...

Satyendra Mondal

Dear Sebastian Hurtado Cortes

Please find the attachment. I hope it will help you to work with the pET system.

Best wishes.