Can someone kindly recommend the catalog number of metformin for oral use?

05 July 2020 0 8K Report

I want to add metformin to the mice's drinking water, but there are a lot of forms of soluble metformin in the Sigma-Aldrich website. I will be grateful for the recommended form.

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Is a 5` phosphate needed for homologous recombination?

I am a bit confused whether phosphorylated DNA is needed to achieve efficient homologous recombination in e coli. I believe there are methods that use ssDNA oligos or PCR products as inserts for...

04 January 2024 2,196 1 View

Determining lagging DNA strand for lambdaphage recombineering?

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16 August 2023 7,660 4 View

Can JM109(DE3) be used for blue-white screening?

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21 June 2023 3,398 2 View

Can Griess reagent detact NO in brain samples?

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12 June 2023 7,829 1 View

Recommendations for PSMA expressing cell line?

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08 June 2022 9,383 2 View

What is the rationale behind Lambda phage gene order?

If I understand the Lambda genome correctly it seems that some of the sequence of genes isn't the most logical. When the phage enters the lytic cycle its late rightward promoter first transcribes...

23 March 2022 10,025 2 View

Can MCE filters be used to bind protein?

I am trying a new protocol to screen colonies for protein expression where a membrane is placed on the plated colonies and lifted. Then the adsorbed cells are lysed and the protein binds to the...

22 February 2022 9,179 2 View

Can antibodies to phage lambda can be purchased?

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29 November 2020 3,921 2 View

How to get bacteriophage lambda virulent mutants?

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09 September 2020 9,653 1 View

Any ideas what is going wrong with my bacteriophage plaque assays?

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25 August 2020 2,164 6 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

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08 August 2024 9,157 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

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06 August 2024 1,989 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

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06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

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04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

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04 August 2024 762 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

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03 August 2024 4,725 2 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

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03 August 2024 2,730 1 View