Can someone kindly help me with details, website where I can buy a kit for Yellow Fever Virus IgM and/or IgG ELISA?

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Do you have expertise on use of @ RISK?

Looking for collaboration on risk analysis, if you are skilled in using @RISK and interested, kindly send me a personal mail [email protected]

06 July 2018 5,413 3 View

How sensitive are mice to dengue virus?

I want to isolate/build the titre of dengue virus in mice, The mosquitoes were caught and stored at -56°C for a long time. Please who has experience in the technique of mice inoculation with...

08 September 2014 4,589 8 View

Can anyone help with troubles relating to the loading tray of real time PCR?

After using the qPCR days before for a multiplex reaction, I created another run template for a singleplex reaction and set the thermal profile. As I started the reaction, an error message from...

07 August 2014 3,123 3 View

How can I get rid of unspecific binding in conventional PCR?

I obtained very sharp bands in the region I am amplifying, but there are some faint bands below still (I guess primer dimers). I raised and lowered the temperature by one degree, for the +1degree...

07 August 2014 567 7 View

Why am I unable to get a PCR band size i got earlier?

I used a protocol to amplify a segment of a viral RNA. After making cDNA, I was able to detect the positive control four times with excellent bands, each time i used the same reagents including...

07 August 2014 7,986 8 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View