Hi friends, a question for oocyte experts.
I assume that the area marked in red is the GV. Am I correct? If so, what is the area outlined in blue? Is it also a part of the GV?
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
I have a problem, I can't share the file of my article. An error message appears, please help.
17 December 2023 8,453 0 View
Dear colleagues, the images show fibrous material after 1h contact with whole blood. Samples where washed with PBS and prepared for SEM observations: fixing with PFA, dehydration with graded...
08 November 2023 7,438 3 View
Hi friends, I have a question about my fibroblasts cell culture. Lately they have been looking different than usual. As you can see in the photo (second one), the cytoplasm appears to be much...
04 October 2023 1,203 3 View
I am running an experimental research design. If I want a run my analyses with only a particular demographic of participants in my sample (e.g. just men). Should I use the select cases function...
17 April 2023 7,402 3 View
For context, the study I am running is a between-participants vignette experimental research design. My variables include: 1 moderator variable: social dominance orientation (SDO) 1 IV: target...
11 April 2023 6,091 9 View
Hi, currently I'm working on modelling the viscoplastic behaviour of solid materials by using the discrete element method. I want to use the scaling method to improve the computational time. Do...
09 March 2023 9,815 6 View
Specifically, I mean how is it working, for example when someone that builds a house, wants to change colours, proportions, ect, things that are basically prescribe on the legislative level. Is it...
23 November 2022 5,448 2 View
I am looking for good practices in the area of the commercialisation of R&D results. I am interested in the commercialisation models used by R&D organisations that are involved both in...
25 May 2021 644 8 View
In my recent study I had one independent variable (IV, 2 levels) and a number of different explanatory variables and one response. The aim was to assess the relationship between teamwork...
13 August 2020 4,433 6 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View