I have been reading many papers but struggling to find a clear explanation for how to interpret the data apart from lower FP - faster tumbling, higher FP slower tumbling. I've not been taught on this topic, but have been given data to analyse as part of my honours project.
The data is on florescence polarisation with heparin, I also have random spikes in data, where FP is reading low at a certain concentration of heparin but one replicate will read a very high FP from the rest of the replicates in that concentration, is there an explanation for this?
Any help would be greatly appreciated. Thankyou.
luorescence polarization (FP) is a technique used to measure the rotation of a fluorescent molecule in a solution. The principle of FP is that a molecule with a larger molecular weight will rotate more slowly and have a higher FP value, while a molecule with a smaller molecular weight will rotate more quickly and have a lower FP value.
When interpreting FP data, there are a few key things to consider:
Overall, interpreting FP data requires a combination of understanding the underlying principles of the technique, careful consideration of the experimental conditions, and appropriate data analysis and quantitation. It's always a good idea to consult with a mentor or experienced researcher in the field to ensure that you're interpreting the data correctly.
- Badges
- Science topic
- Similar topics
- Philosophy
- Esthetics
- Interpretation