Determine the number of quails in a treatment/replication/pen?

How can we estimate the number of quail birds in a treatment and in a replication for 5 treatment and 4 replication for feed research? Is there any range limitation to keep the number of birds?

19 November 2020 2,856 3 View

Does any one know an integrated cloud governance framework for all levels of government (federal/state or provincial, and municipal)? Any suggestions?

How can the Social Cost Benefit-SCB Analysis be used in Cloud adoption? Perhaps, it is equally important as the Return on Investment-ROI?

04 September 2020 9,310 1 View

How it is possible that DNA primers previously working suddenly stop to worked?

I have used the same exact sequences in primers while ordering the new primers. The old primers are that I used previously which was worked successfully. Similarly, the sequences in tubes are also...

20 August 2020 3,132 12 View

Can you suggest some unconjugated primary antibodies for IHC against Pig formalin-fixed paraffin embedded(FFPE) tissue samples?

I am looking for unconjugated primary antibodies against CD3,CD4,CD8,CD11c, CD14, CD21,IgM, MHC-II in Pig FFPE tissue samples. Has anyone used Pig FFPE tissues for IHC against these markers ?

08 December 2019 1,450 3 View

Is it possible to perform Mann-Kendall Test for trend analysis of 5 years time series data?

I am having confusion and problem in trend analysis. Is it possible to perform Mann-Kendall test for trend analysis of 5 years time series data of groundwater level?

08 October 2019 733 4 View

Why PCR from cDNA is not working?

We are trying to clone a group of genes but failed at PCR step. What could be the possible reason? our protocol is briefly outlined below: 1. Extract total RNA from HEK293 cells (purity and...

07 May 2019 1,813 5 View

How is the processing of ERP signals done ?

I have collected 2 channels of EEG data with subjects looking at some visual stimulus. I have looked into a bunch of websites regarding EEG processing steps once we acquire the signal. But I have...

10 February 2019 5,989 5 View

Is there any oddball paradigm experiments written in matlab ?

I want to use oddball experiment for my EEG research. Can anyone provide any code which uses such oddball paradigm ?

10 February 2019 9,244 2 View

Is A4/Fuk cell lines metabolically active?

I have cultured A4/Fuk cell lines and it looked healthy in T75 flasks. I seeded for drug screening and growth curve. The cell count in growth curve in next day showed that there is no growth of...

06 February 2019 406 3 View

Can we do regression analysis in SPSS using the imputed data from measurement model in AMOS?

Dear Scholars I am going to test a complex model (11 latent constructs with 65 items). I did pooled measurement model in AMOS and reached good model fit with the help of modification indices. Out...

16 November 2018 6,347 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View