Can somebody suggest a software package for deconvolution of fluorescence decay from the IRF used for calculating fluorescence lifetime?

More Saurabh Chauhan's questions See All

Can somebody help with steady state emission data of CdSe-QDs+MO2 (M=Ti or Zr) solutions?

I am doing some control experiments with CdSe QDs-MO2 (M=Ti or Zr) colloidal solutions. According to literature, I should see lesser quenching of emission with ZrO2 as compared to TiO2 as the band...

01 February 2015 4,728 0 View

How can I measure the longer fluorescence lifetime component of quantum dots using TCSPC?

Hello, I am using a Becker and Hickl Tau130 TCSPC system to calculate fluorescence lifetimes of CdSe quantum dots. With me early measurements I have observed that QDs show multi-exponential decay...

08 September 2014 399 13 View

Can somebody suggest an ultra short fluorescence lifetime standard which absorbs light at 440 nm?

I am using a Beckr and Hickl Tau 130 TCSPC setup for fluorescence lifetime measurement of different fluorophores. For excitation, I am using a pulsed laser (440 nm). For now, I am using...

07 August 2014 4,014 5 View

Can somebody suggest a standard data analysis software package for time resolved fluorescence experiments for calculation of lifetimes?

I am using a Becker and Hickl Tau 130 - single time correlated photon counting (TCSPC) instrumentation to collect data. Thanks!

06 July 2014 6,930 12 View

Can anyone help with measuring IRF (instrument response function) for fluorescence lifetime experiment by TCSPC.

I have been using water to scatter light for measuring IRF of my TCSPC setup. I have also try to use paper. But when I was searching online, I saw that people generally use colloidal silica/ ludox...

05 June 2014 5,126 11 View

Can anyone give some advice on TCSPC setup for fluorescence lifetime experiment?

Setup (refer attachment):diode laser (440nm, polarization-horizontal), sample, detection of fluporescence at 90 degrees from excitation, fluorescence collecting planoconvex lens (1 inch diameter),...

05 June 2014 1,191 5 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

Richard A. Murray

You could try Fluofit from Picoquant but I'm not sure if you can use it without a valid license.

You could also try FLIMfit which was developed by the guys over at Imperial College in London. I've attached a link below for FLIMfit

http://downloads.openmicroscopy.org/flimfit/4.6.6/

Boguslaw Szczupak

Would also recommend FAST software from Edinburgh Instruments

http://www.edinburghphotonics.com/spectrometers/software/fast/

M. Eugenio Vazquez

Haven't used this one yet, but looks really nice

http://www.fluortools.com/software/decayfit

good luck!