I have surface modified my slides with silane and want to quantify the amine groups by using FITC.
Also, suggest ways to remove excessive dye.
Hello,
Use DMF as a solvent with FITC and label your sample with it by allowing proper mixing of the two for 10-12 h.
Concentrate the mixed samples over vacuum. Wash with ethyl acetate at least thrice to remove the excess FITC.
Hope it works.
Thanx Ayushi.
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