What could be possible reason of higher Drug Concentration of Liposomal formulation stability samples over time?

Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...

25 January 2021 7,752 4 View

What is the advantage of DFT over tight-binding methods or vice-versa?

It might be a very basic question, but can somebody please put some light that what is the advantage of using Density Functional Theory (DFT) over tight-binding approximation or vice-...

31 August 2020 4,937 3 View

How the position of band inversion (near or away the Fermi level) affects the electrical transport and thermal properties in semi-metals?

Dear all, Can somebody please put some light on how the position of band inversion, near or away the Fermi level affects the electrical transport and thermal properties in semi-metals? Thanks in...

19 August 2020 5,202 8 View

My protein is a recombinant protein constitutively expressed in Pichia.Why am I getting a smear/ dragging of the loaded protein?

The protein (55KDa) has low contentration. It is purified via ammonium sulphate salting out and desalted with pD10 column. Will working with SDS-PAGE (10% resolving and 5% stacking), a drag is...

05 June 2019 4,216 14 View

How to perform band structure calculations with HSE + SOC in VASP?

Can Somebody please tell me that how to perform band structure calculations with HSE + spin-orbit coupling (SOC) in VASP? In normal HSE calculations, we perform SCF claculations with WAVECAR of...

07 January 2019 1,310 6 View

What is the meaning of parity of a band?

As we know, a band has partial contribution from all the orbitals of all the atoms, then how can we determine the parity of a band from these partial contributions? What is the physics involved in...

22 February 2018 5,256 8 View

Is there any theory which explains the origin of the existence of indirect band gap for a material?

As we know, Kronig Penney model explains the existence of band gap at the same 'k', i.e. direct band gap. However, there is nothing like indirect band gap in KP model. Then, how can we explain the...

02 May 2017 7,619 3 View

What are High symmetry points in a crystal?

12 February 2017 6,848 7 View

PSI Short form Abidin scoring?

Can anyone please guide me about how to interpret the subscale scores for the Parenting Stress Index (Esp the parental Dysfunctional subscale). Any guidance will be much appreciated!!!

18 January 2017 2,828 3 View

Any ideas of how to run a power analysis to determine sample size for a within group study?

Hi I am planning to run a within group study (collecting data from the same participants thrice). The questionnaires I am using are the BRIEF COPE, PSI (Parental stress index) and PSS (perceived...

13 November 2016 3,357 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Can anyone suggest me broad host range fluorescence expression vectors for Gram-positive bacteria ?

Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...

06 February 2021 2,566 4 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

How to show a protein is being dimerized in SDS-PAGE gel?

A protein dimerizes through CTD. I have expressed CTD of protein with two different tags. Now, I want to show that these CTD of protein dimerize in a pull-down assay. But my doubt is that if the...

29 January 2021 7,529 4 View

How to solubilize protein?

Hello, I have cloned my gene of interest in a bacterial expression system i.e. BL 21 Gold (DE3) and checked for protein production and solubility. Good amount of protein was observed on SDS-PAGE...

15 January 2021 7,063 5 View