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Can we block unwanted images during the x-ray prediction?

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I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

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Do I need to strip the PVDF membrane before staining it with colloidal gold?

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Do you know any MR image dataset?

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How to find the mode of classified ocean colour images?

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How to threshold an image despite bright lighting, using ImageJ/Fiji?

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Trouble with Charting with Google Earth Engine (Technical)?

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Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

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Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

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Fiona Cunningham

Hi Zahida, did you ever find a solution to this problem? I am currently investigating how to do something similar myself.

Sara Parker

What I wrote in my dissertation for how I quantified recovery after calcium switch assays: Percent recovery of junctional occludin/ZO-1 was measured by thresholding to the same percent of histogram, and the Skeletonize plug-in (Arganda-Carreras et al. 2010) was used to reduce signal to 1 pixel wide segments. Length of segments were measured, summed, and divided by total length of the cell junction to give percent recovery.

I only quantified the junctions between, in my case, two cells expressing the plasmid of interest.

Hope that helps!